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Overview
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Product name
Anti-Mu Opioid Receptor antibody [UMB3] - BSA and Azide free
See all Mu Opioid Receptor primary antibodies -
Description
Rabbit monoclonal [UMB3] to Mu Opioid Receptor - BSA and Azide free
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Host species
Rabbit
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Specificity
The antibody detects the band of interest in transfected cell lines. When tested in our lab, we were not able to detect any signal from the endogenous protein.
This antibody can be used in IHC and ICC/IF as demonstrated in several publications, however, it did not pass our internal validation in these applications. Please refer to PMID 20851148, 21957251, 26290245, and other references.
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Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt -
Species reactivity
Reacts with: Recombinant fragment
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mu Opioid Receptor transfected HEK-293 lysate.
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General notes
ab271900 is the carrier-free version of ab134054.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid
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Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze.
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Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes
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Concentration information loading...
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Purity
Protein A purified
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Clonality
Monoclonal
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Clone number
UMB3
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Isotype
IgG
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Research areas
- Neuroscience
- Neurotransmission
- Receptors / Channels
- GPCR
- Opioid Receptors
- Neuroscience
- Sensory System
- Somatosensory system
- Nociception
- Cell Biology
- Other Antibodies
- Drugs and Chemicals
- Neuroscience
- Processes
Associated products
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Alternative Versions
- Anti-Mu Opioid Receptor antibody [UMB3] - C-terminal (ab134054)
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Compatible Secondaries
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Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab271900 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | Use at an assay dependent concentration. Detects a band of approximately 75-78 kDa (predicted molecular weight: 45 kDa). |
Notes |
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WB |
Application notes
Is unsuitable for Flow Cyt.
Target
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Function
Inhibits neurotransmitter release by reducing calcium ion currents and increasing potassium ion conductance. Receptor for beta-endorphin.
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Sequence similarities
Belongs to the G-protein coupled receptor 1 family.
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Cellular localization
Cell membrane.
- Information by UniProt
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Database links
- Entrez Gene: 18390 Mouse
- Entrez Gene: 25601 Rat
- SwissProt: P42866 Mouse
- SwissProt: P33535 Rat
- Unigene: 457998 Mouse
- Unigene: 10118 Rat
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Alternative names
- hMOP antibody
- LMOR antibody
- M-OR-1 antibody
see all
Images
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Western blot - Anti-Mu Opioid Receptor antibody [UMB3] - BSA and Azide free (ab271900)
All lanes : Anti-Mu Opioid Receptor antibody [UMB3] - C-terminal (ab134054) at 1/1000 dilution
Lane 1 : Mu Opioid Receptor transfected HEK-293 (Human epithelial cell line from embryonic kidney) lysate
Lane 2 : HEK-293T cell lysateLysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilutionPredicted band size: 45 kDa
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Anti-Mu Opioid Receptor antibody [UMB3] - BSA and Azide free (ab271900)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Click here to view the general protocols
Datasheets and documents
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Datasheet download
Download
Certificate of Compliance
References (0)
Publishing research using ab271900? Please let us know so that we can cite the reference in this datasheet.
ab271900 has not yet been referenced specifically in any publications.