Recombinant Anti-Mu Opioid Receptor antibody [UMB3] (2024)

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Recombinant Anti-Mu Opioid Receptor antibody [UMB3] (1)

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Overview

  • Product name

    Anti-Mu Opioid Receptor antibody [UMB3] - BSA and Azide free
    See all Mu Opioid Receptor primary antibodies

  • Description

    Rabbit monoclonal [UMB3] to Mu Opioid Receptor - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    The antibody detects the band of interest in transfected cell lines. When tested in our lab, we were not able to detect any signal from the endogenous protein.

    This antibody can be used in IHC and ICC/IF as demonstrated in several publications, however, it did not pass our internal validation in these applications. Please refer to PMID 20851148, 21957251, 26290245, and other references.

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt

  • Species reactivity

    Reacts with: Recombinant fragment
    Predicted to work with: Mouse, RatRecombinant Anti-Mu Opioid Receptor antibody [UMB3] (2)

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mu Opioid Receptor transfected HEK-293 lysate.
  • General notes

    ab271900 is the carrier-free version of ab134054.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production

    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes

  • Recombinant Anti-Mu Opioid Receptor antibody [UMB3] (3)Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    UMB3

  • Isotype

    IgG

  • Research areas

    • Neuroscience
    • Neurotransmission
    • Receptors / Channels
    • GPCR
    • Opioid Receptors
    • Neuroscience
    • Sensory System
    • Somatosensory system
    • Nociception
    • Cell Biology
    • Other Antibodies
    • Drugs and Chemicals
    • Neuroscience
    • Processes

Associated products

  • Alternative Versions

    • Anti-Mu Opioid Receptor antibody [UMB3] - C-terminal (ab134054)
  • Compatible Secondaries

  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab271900 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB

Use at an assay dependent concentration. Detects a band of approximately 75-78 kDa (predicted molecular weight: 45 kDa).

Notes

WB
Use at an assay dependent concentration. Detects a band of approximately 75-78 kDa (predicted molecular weight: 45 kDa).

Application notes

Is unsuitable for Flow Cyt.

Target

  • Function

    Inhibits neurotransmitter release by reducing calcium ion currents and increasing potassium ion conductance. Receptor for beta-endorphin.

  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family.

  • Cellular localization

    Cell membrane.

  • Information by UniProt
  • Database links

  • Alternative names

    • hMOP antibody
    • LMOR antibody
    • M-OR-1 antibody

    see all

Images

  • Western blot - Anti-Mu Opioid Receptor antibody [UMB3] - BSA and Azide free (ab271900)

    All lanes : Anti-Mu Opioid Receptor antibody [UMB3] - C-terminal (ab134054) at 1/1000 dilution

    Lane 1 : Mu Opioid Receptor transfected HEK-293 (Human epithelial cell line from embryonic kidney) lysate
    Lane 2 : HEK-293T cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 45 kDa

  • Anti-Mu Opioid Receptor antibody [UMB3] - BSA and Azide free (ab271900)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

Certificate of Compliance

References (0)

Publishing research using ab271900? Please let us know so that we can cite the reference in this datasheet.

ab271900 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Recombinant Anti-Mu Opioid Receptor antibody [UMB3] (6)

Recombinant Anti-Mu Opioid Receptor antibody [UMB3] (2024)
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